Use of tritiated prostaglandins in metabolism studies. II: Kinetic isotope effect: an useful tool to investigate catabolizing sequence of prostaglandins

Journal Article · Sat Mar 01 04:00:00 EST 1986 · Prostaglandins; (United States)

DOI:https://doi.org/10.1016/0090-6980(86)90112-7· OSTI ID:5203192

Moussard, C; Alber, D; Perruche, C; Henry, J C

It is well established that prostaglandin catabolism involves sequential actions of a 15-hydroxyprostaglandin dehydrogenase, a 15-keto-prostaglandin delta 13-reductase and a 15-ketoprostaglandin reductase. This pathway must be confirmed in never investigated tissues before any enzyme assay is carried out. We have developed a new, simple, rapid and reliable method to investigate catabolizing sequence of prostaglandins based on the tritium kinetic isotope effect which occurs during the oxidation of the 15-hydroxyl group of the prostaglandin into a 15-keto group.

• Research Organization: Universite de Franche Comte, Besancon, France
• OSTI ID: 5203192
• Journal Information: Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 3; ISSN PRGLB
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
CHEMISTRY
DIGESTIVE SYSTEM
ENZYMES
GLANDS
GUINEA PIGS
IN VITRO
ISOTOPE EFFECTS
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
ORGANS
OXIDOREDUCTASES
PROSTAGLANDINS
RADIONUCLIDE KINETICS
RATS
RODENTS
TRITIUM COMPOUNDS
VERTEBRATES