Use of tritiated prostaglandins in metabolism studies. I: Evaluation of the kinetic isotope effect in the prostaglandin dehydrogenase reactions

Journal Article · Sat Mar 01 04:00:00 EST 1986 · Prostaglandins; (United States)

DOI:https://doi.org/10.1016/0090-6980(86)90111-5· OSTI ID:5230718

Moussard, C; Alber, D; Perruche, C; Henry, J C

Although numerous data exist concerning tritium kinetic isotope effect in enzymic reactions, little is related to the metabolism of tritiated prostaglandins. The present study reports an evaluation of the kinetic isotope effect which occurs during the oxidation of 15-hydroxyl group of tritium-labeled prostaglandins E2 and F2 alpha by the 15-hydroxyprostaglandin dehydrogenase and during the oxidation of 9-hydroxyl group of tritium-labeled prostaglandin F2 alpha by the 9-hydroxyprostaglandin dehydrogenase. The large kinetic isotope effect tends to limit the validity of the dehydrogenase assay using tritium-labeled prostaglandins as substrate. However these assays can be considered to be an indication of relative enzyme activity.

• Research Organization: Universite de Franche-Comte, Besancon, France
• OSTI ID: 5230718
• Journal Information: Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 3; ISSN PRGLB
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ENZYME ACTIVITY
ENZYMES
FETAL MEMBRANES
GUINEA PIGS
IN VITRO
ISOTOPE EFFECTS
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
METABOLISM
OXIDOREDUCTASES
PLACENTA
PREGNANCY
PROSTAGLANDINS
RADIONUCLIDE KINETICS
RATS
RODENTS
TRITIUM COMPOUNDS
VERTEBRATES