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Radioimmunoassay for. gamma. -melanocyte stimulating hormone

Journal Article · · Life Sci.; (United States)
A specific radioimmunoassay for ..gamma..-melanocyte stimulating hormone-like peptides was developed. An antiserum raised in rabbit to synthetic bovine ..gamma../sub 3/-MSH, one of the possible ..gamma..-MSH peptides, specifically recognizes the portion between His/sup 5/ and Arg/sup 14/ of ..gamma../sub 3/-MSH without significant cross-reaction with other synthetic ..gamma..-MSH-like peptides, ..cap alpha..-, ..beta..-MSH, adrenocorticotropin, and ..beta..-endorphin. The usable range of this RIA is 10 pg to 600 pg of synthetic ..gamma../sub 3/-MSH. Three immunoreactive ..gamma..-MSH peaks were thus found in gel permeation chromatography of the whole bovine pituitary extract.
Research Organization:
Salk Inst. for Biological Studies, La Jolla, CA
OSTI ID:
5180017
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 26:21; ISSN LIFSA
Country of Publication:
United States
Language:
English