Radioimmunoassay of CRF-like material in rat hypothalamus
Journal Article
·
· Peptides (Fayetteville, N.Y.); (United States)
Corticotropin releasing factor (CRF) was recently isolated from ovine hypothalami by its ability to stimulate adrenocorticotropin (ACTH) and beta-endorphin release from dispersed rat pituitary cells. In order to study the physiology of this peptide, we have developed a sensitive and specific radioimmunoassay (RIA) for CRF. Synthetic CRF was conjugated to bovine thyroglobulin and emulsified with complete Freund's adjuvant. A suitable antiserum was obtained which showed no crossreactivity with eight naturally occurring peptides. N-Tyr-CRF was iodinated and used as tracer. With this assay, CRF-like immunoreactivity which coeluted with ovine CRF on Sephadex G50 was detected in rat hypothalami.
- Research Organization:
- New York University Medical Center, New York
- OSTI ID:
- 6816633
- Journal Information:
- Peptides (Fayetteville, N.Y.); (United States), Journal Name: Peptides (Fayetteville, N.Y.); (United States) Vol. 3:1; ISSN PPTDD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Corticotropin releasing factor (CRF): immunocytochemical localization and radioimmunoassay (RIA). [Rats]
A specific radioimmunoassay for corticotropin releasing factor (CRF) using synthetic ovine CRF. [Rats]
beta. -lipotropin is the major opioid-like peptide of human pituitary and rat pars distalis: lack of significant. beta. -endorphin
Journal Article
·
Sun Nov 28 23:00:00 EST 1982
· Life Sci.; (United States)
·
OSTI ID:6516666
A specific radioimmunoassay for corticotropin releasing factor (CRF) using synthetic ovine CRF. [Rats]
Journal Article
·
Sun Feb 27 23:00:00 EST 1983
· Life Sci.; (United States)
·
OSTI ID:5917051
beta. -lipotropin is the major opioid-like peptide of human pituitary and rat pars distalis: lack of significant. beta. -endorphin
Journal Article
·
Thu Jun 01 00:00:00 EDT 1978
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6688316
Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BIOCHEMISTRY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMISTRY
ENDOCRINE GLANDS
GLANDS
HORMONES
HYPOTHALAMUS
IMMUNE SERUMS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NERVOUS SYSTEM
ORGANS
PEPTIDE HORMONES
PHYSIOLOGY
PITUITARY GLAND
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RATS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BIOCHEMISTRY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHEMISTRY
ENDOCRINE GLANDS
GLANDS
HORMONES
HYPOTHALAMUS
IMMUNE SERUMS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NERVOUS SYSTEM
ORGANS
PEPTIDE HORMONES
PHYSIOLOGY
PITUITARY GLAND
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RATS
RODENTS
TRACER TECHNIQUES
VERTEBRATES