Corticotropin releasing factor (CRF): immunocytochemical localization and radioimmunoassay (RIA). [Rats]

Journal Article · Mon Nov 29 04:00:00 EST 1982 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(82)90748-2· OSTI ID:6516666

Vigh, S ^[1]; Merchenthaler, I; Torres-Aleman, I; Sueiras-Diaz, J; Coy, D; Carter, W H; Petrusz, P; Schally, A V

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1. Tulane Univ. School of Medicine, New Orleans, LA

Two fragments of the amino acid sequence corresponding to ovine corticotropin releasing factor (CRF 37-41 and CRF 22-41), as well as the full sequence of 41 residues (CRF 1-41), synthesized in our laboratories by solid-phase methods, were coupled to bovine serum albumin (BSA) with glutaraldehyde. New Zealand white rabbits were immunized with the emulsified mixtures of peptide-BSA conjugates and Freund's adjuvant as immunogens. The specificity of the generated antibodies was studied by agar-gel diffusion, absorption tests in the immunohistochemical system, and with the aid of displacement curves in RIA. /sup 125/I-Tyr(35)-CRF 36-41 and /sup 125/I-Tyr(0)-CRF 1-41 were used as radioligands in the RIA. The minimum detectable dose was 20 pg. The linearity observed in RIA for immunoreactive CRF in extracts of rat hypothalami, together with the immunocytochemical findings in the rat brain, indicate the presence of substance(s) immunologically indistinguishable from CRF. Immunohistochemistry with the peroxidase-antiperoxidase (PAP) technique detected the following CRF-immunoreactive structures in vibratome sections of hypothalami of colchicine-treated rats: CRF-containing cell bodies were observed mainly in smaller neurons of the paraventricular nucleus. CRF-positive nerve fibers and/or terminals were present in the external zone of the median eminence, with some immunoreactive CRF also present in the internal zone. The CRF-positive terminals were localized in the central regions of the median eminence. Data reinforce the view that this polypeptide plays a physiological role in the control of ACTH release.

• OSTI ID: 6516666
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 31:22; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACTH
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BODY
BRAIN
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
HYDROXY ACIDS
HYPOTHALAMUS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGANDS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
REACTION KINETICS
TRACER TECHNIQUES
TYROSINE