Distribution and characterization of two non-opiod peptides with morphine modulating activity in brain

Journal Article · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6859649

Majane, E A; Yang, H Y.T.

Two peptides which are immunoreactive to Phe-Met-Arg-Phe-NH/sub 2/ (FMRF-NH/sub 2/) antiserum were purified from bovine brain, chemically characterized and synthesized. These two peptides, octapeptide octadecapeptide, can reduce rat tail flick latencies; octapeptide can also attenuate morphine induced analgesia. The distribution of these two peptides in brain was studied by radioimmunoassay. Octapeptide antiserum shows high affinity for synthetic octapeptide, PYY and FMRF-NH/sub 2/. The antiserum is almost totally inactive to ..gamma../sub 1/-MSH. Octadecapeptide antiserum shows high affinity for octadecapeptide, some cross-reaction for the octapeptide (50%), ..gamma../sub 1/-MSH (10%) and FMRF-NH/sub 2/ (0.01%). NPY, PYY, Met/sup 5/-Enk-Arg-Phe and CCk are inactive. Octa and Octadecapeptide immunoreactivities are unevenly distributed in bovine brain. The highest concentrations (pmol g/sup -1/) are found in dorsal spinal cord (9.8 and 16.4 respectively), periaqueductal gray (8.6 and 6.8) and pons medulla (7.0 and 8.9); non-detectable amounts in cortex and cerebellum. The enrichment of these peptides in dorsal cord and PAG, areas important in opoid-mediated pain perception, suggest that they may play a role in mediating antinociception. Furthermore, using these two antisera, it is now possible to differentiate mammalian FMRF-NH/sub 2/-like and NPY.

• Research Organization: St. Elizabeths Hospital, Washington, DC
• OSTI ID: 6859649
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ALKALOIDS
ANALGESICS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CATTLE
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMICAL COMPOSITION
DISTRIBUTION
DOMESTIC ANIMALS
DRUGS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MORPHINE
NARCOTICS
NERVOUS SYSTEM
OPIUM
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
PURIFICATION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
RUMINANTS
SPINAL CORD
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES