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Title: Direct /sup 125/I-radioligand assays for serum progesterone compared with assays involving extraction of serum

Abstract

Two direct radioimmunoassays for progesterone in 50 ..mu..L of unextracted serum or plasma with assays involving extraction of serum were compared. The direct assays include the use of either danazol at pH 7.4 or 8-anilino-1-naphthalenesulfonic acid at pH 4.0 to displace progesterone from serum binding-proteins. Progesterone is then assayed by using an antiserum to a progesterone 11..cap alpha..-hemisuccinyl conjugate and the radioligand /sup 125/I-labeled progesterone 11..cap alpha..-glucuronyl tyramine, with separation by double-antibody techniques. Direct assays with either displacing agent gave good analytical recovery of progesterone added to human serum, and progesterone values for patients' specimens correlated well (r > 0.96) with results of assays involving extraction of serum. Precision was similar with each displacing agent over the working range 2.5-100 nmol/L and superior to that of extraction assays. We conclude that these direct assays of progesterone are analytically valid and more robust, precise, and technically convenient than many conventional methods involving extraction of serum.

Authors:
 [1]; ; ;
  1. Glasgow Royal Infirmary, United Kingdom
Publication Date:
OSTI Identifier:
5176794
Resource Type:
Journal Article
Journal Name:
Clin. Chem. (Winston-Salem, N.C.); (United States)
Additional Journal Information:
Journal Volume: 28:6
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PROGESTERONE; RADIOIMMUNOASSAY; BLOOD PLASMA; BLOOD SERUM; IODINE 125; LABELLED COMPOUNDS; PATIENTS; TRACER TECHNIQUES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KETONES; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PREGNANES; RADIOASSAY; RADIOISOTOPES; STEROID HORMONES; STEROIDS; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Ratcliffe, W A, Corrie, J E.T., Dalziel, A H, and Macpherson, J S. Direct /sup 125/I-radioligand assays for serum progesterone compared with assays involving extraction of serum. United States: N. p., 1982. Web.
Ratcliffe, W A, Corrie, J E.T., Dalziel, A H, & Macpherson, J S. Direct /sup 125/I-radioligand assays for serum progesterone compared with assays involving extraction of serum. United States.
Ratcliffe, W A, Corrie, J E.T., Dalziel, A H, and Macpherson, J S. 1982. "Direct /sup 125/I-radioligand assays for serum progesterone compared with assays involving extraction of serum". United States.
@article{osti_5176794,
title = {Direct /sup 125/I-radioligand assays for serum progesterone compared with assays involving extraction of serum},
author = {Ratcliffe, W A and Corrie, J E.T. and Dalziel, A H and Macpherson, J S},
abstractNote = {Two direct radioimmunoassays for progesterone in 50 ..mu..L of unextracted serum or plasma with assays involving extraction of serum were compared. The direct assays include the use of either danazol at pH 7.4 or 8-anilino-1-naphthalenesulfonic acid at pH 4.0 to displace progesterone from serum binding-proteins. Progesterone is then assayed by using an antiserum to a progesterone 11..cap alpha..-hemisuccinyl conjugate and the radioligand /sup 125/I-labeled progesterone 11..cap alpha..-glucuronyl tyramine, with separation by double-antibody techniques. Direct assays with either displacing agent gave good analytical recovery of progesterone added to human serum, and progesterone values for patients' specimens correlated well (r > 0.96) with results of assays involving extraction of serum. Precision was similar with each displacing agent over the working range 2.5-100 nmol/L and superior to that of extraction assays. We conclude that these direct assays of progesterone are analytically valid and more robust, precise, and technically convenient than many conventional methods involving extraction of serum.},
doi = {},
url = {https://www.osti.gov/biblio/5176794}, journal = {Clin. Chem. (Winston-Salem, N.C.); (United States)},
number = ,
volume = 28:6,
place = {United States},
year = {Tue Jun 01 00:00:00 EDT 1982},
month = {Tue Jun 01 00:00:00 EDT 1982}
}