Characterization of the phosphatidylinositol-glycan membrane anchor of human placental alkaline phosphatase

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.17.6055· OSTI ID:5175508

Howard, A D; Berger, J; Gerber, L; Familletti, P; Udenfriend, S

Placental alkaline phosphatase (orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1) is a member of a diverse group of membrane proteins whose attachment to the lipid bilayer is mediated by a phosphatidylinositol-glycan. To investigate structural aspects of the glycolipid anchor, cultured WISH cells were used because, they produce the enzyme in abundant quantities. When cell suspensions were incubated with purified phosphatidylinositol-specific phospholipase C, most of the placental alkaline phosphatase was released from membranes in a hydrophilic form. On incubation of the cells with (/sup 14/C)ethanolamine, (/sup 14/C)myristic acid, or myo(/sup 3/H)inositol, each was incorporated into the phosphatase near the carboxyl terminus, showing that these components, which are found in other phosphatidylinositol membrane-linked proteins, are also present in placental alkaline phosphatase.

• Research Organization: Roche Research Center, Nutley, NJ (USA)
• OSTI ID: 5175508
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:17; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
AMINES
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMISTRY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
ENZYMES
ESTERASES
EVEN-ODD NUCLEI
FETAL MEMBRANES
GLYCOLIPIDS
HYBRIDIZATION
HYDROLASES
INOSITOLS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
MEMBRANES
MESSENGER-RNA
METHIONINE
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHATASES
PLACENTA
RADIOISOTOPES
RNA
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TETRADECANOIC ACID
TRITIUM COMPOUNDS