Phospholipase D specific for the phosphatidylinositol anchor of cell-surface proteins is abundant in plasma

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.4.980· OSTI ID:6659453

Low, M G; Prasad, A R.S.

An enzyme activity capable of degrading the glycosyl-phosphatidylinositol membrane anchor of cell-surface proteins has previously been reported in a number of mammalian tissues. The experiments reported here demonstrate that this anchor-degrading activity is also abundant in mammalian plasma. The activity was inhibited by EGTA or 1,10-phenanthroline. It was capable of removing the anchor from alkaline phosphatase, 5'-nucleotidase, and variant surface glycoprotein but had little or no activity toward phosphatidylinositol or phosphatidylcholine. Phosphatidic acid was the only /sup 3/H-labeled product when this enzyme hydrolyzed (/sup 3/H)myristate-labeled variant surface glycoprotein. It could be distinguished from the Ca/sup 2/=-dependent inositol phospholipid-specific phospholipase C activity in several rat tissues on the basis of its molecular size and its sensitivity to 1,10-phenanthroline. The data therefore suggest that this activity is due to a phospholipase D with specificity for glycosylphosphatidylinositol structures. Although the precise physiological function of this anchor-specific phospholipase D remains to be determined, these findings indicate that it could play an important role in regulating the expression and release of cell-surface proteins in vivo.

• Research Organization: Oklahoma Medical Research Foundation, Oklahoma City (USA)
• OSTI ID: 6659453
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:4; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ALKALINE PHOSPHATASE
BIOCHEMISTRY
BIODEGRADATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CALCIUM COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
FETAL MEMBRANES
HYDROLASES
IONS
LABELLED COMPOUNDS
LIPASE
LIPIDS
LIPOPROTEINS
MATERIALS
MEMBRANES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHATASES
PHOSPHOLIPIDS
PLACENTA
PROTEINS
PURIFICATION
TETRADECANOIC ACID
TRITIUM COMPOUNDS