Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase

Journal Article · Sun Mar 01 00:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.5.1234· OSTI ID:6179285

Henthorn, P S; Raducha, M; Edwards, Y H; Weiss, M J; Slaughter, C; Lafferty, M A; Harris, H

A cDNA clone for human adult intestinal alkaline phosphatase (ALP) (orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1) was isolated from a lambdagt11 expression library. The cDNA insert of this clone is 2513 base pairs in length and contains an open reading frame that encodes a 528-amino acid polypeptide. This deduced polypeptide contains the first 40 amino acids of human intestinal ALP, as determined by direct protein sequencing. Intestinal ALP shows 86.5% amino acid identity to placental (type 1) ALP and 56.6% amino acid identity to liver/bone/kidney ALP. In the 3'-untranslated regions, intestinal and placental ALP cDNAs are 73.5% identical (excluding gaps). The evolution of this multigene enzyme family is discussed.

• Research Organization: Univ. of Pennsylvania School of Medicine, Philadelphia
• OSTI ID: 6179285
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALKALINE PHOSPHATASE
AMINO ACID SEQUENCE
DNA SEQUENCING
ADULTS
BIOLOGICAL EVOLUTION
HYBRIDIZATION
INTESTINES
MAN
PHOSPHORUS 32
PLACENTA
RECOMBINANT DNA
AGE GROUPS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
ENZYMES
ESTERASES
FETAL MEMBRANES
GASTROINTESTINAL TRACT
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHATASES
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques