Development of a transplantable mouse myeloid leukemia model system: a preliminary report

Technical Report · Fri Jan 01 04:00:00 EST 1982

OSTI ID:5175186

Au, W W; Luippold, H E; Otten, J A

Research progess has been hindered in some areas of carcinogenesis because of a lack of consistant biological markers that distinguish cancer from normal cells. Cell lines derived from x-ray induced RFM mouse myeloid leukemia were studied for the purpose of first identifying the genetic differences (by chromosome analysis) between leukemic and normal cells and second, developing a transplantable leukemia model system. Among the cell lines analyzed, one of them (MLI) appeared to be useful in development of such a model. In addition to two chromosome abnormalities, cell line MLI also has a large abnormal chromosome which may be useful as a biological marker for these leukemic cells. This marker was identified by banding study to be an isochromosome 8 derived from centromeric translocation of two chromosome 8s. Upon injection of 4 x 10/sup 6/ leukemic cells into RFM mice symptoms of leukemia appeared from 18 days onwards and mice would begin to die of leukemia from 21 days onwards. The presence or absence of the metacentric marker chromosome was the criteria for inequivocal identification of leukemic from co-existing normal cells in these two tissues. The ratio of these cell populations was quantitated and the time-dependent increase of the ratios was indicative of progression of leukemia. Thus, this transplantable myeloid leukemia model system may be useful in studying the proliferation of leukemic cells and the response of the co-existing cells to therapeutic agents. (ERB)

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5175186
• Report Number(s): CONF-820439-2; ON: DE82013456
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL INDICATORS
BIOLOGICAL MODELS
BONE MARROW CELLS
CELL CULTURES
CELL PROLIFERATION
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DISEASES
HEMIC DISEASES
LEUKEMIA
MUTATIONS
MYELOID LEUKEMIA
NEOPLASMS
SOMATIC CELLS
SPLEEN CELLS
TRANSPLANTS
TUMOR CELLS