Cyclophosphamide/x-ray: combined mode preparation for transplantation therapy

Conference · Sat Sep 01 04:00:00 EDT 1979 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

OSTI ID:5236808

Meredith, R ^[1]; Okunewick, J; Shadduck, R; Raikow, R; Brozovich, B; Seeman, P

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1. Allegheny General Hospital, Pittsburgh, PA

Use of total body irradiation (TBI) and/or chemotherapy as a preparation for marrow transplantation in the treatment of leukemia has been only moderately successful in the clinic. Although cyclophosphamide (CY) has shown promise as a marrow ablative agent, leukemia relapses are often found, and optimal therapeutic protocols have not been established. Our transplantation therapy studies of murine leukemia with parental recipients and hybrid donors provide an excellent model for research aimed at improved survival of human transplant patients. Utilizing a murine leukemia induced by a virus, various doses of CY in combination with sub-lethal irradiation were compared to determine the optimal pretreatment for transplantation therapy. Both normal and leukemic mice were engrafted with virus resistant, histocompatible marrow following these preparations, then monitored for survival and long term effects. Leukemic mice were also evaluated for pluripotent as well as myeloid committed stem cells as a measure of the effectiveness of the treatment in elimination of leukemic cells. Leukemic groups were also compared for the percentage and time of leukemia relapse. All CY/X-ray combinations were more effective in elimination of stem cell populations than supralethal TBI alone. However, the best survival was obtained with lethal TBI alone or low dose CY in combination with 550 R.

• OSTI ID: 5236808
• Conference Information: Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Journal Volume: 5:9
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
DATA
DATA FORMS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
ENDOXAN
EXPERIMENTAL DATA
EXTERNAL IRRADIATION
GRAFT-HOST REACTION
HEMATOPOIETIC SYSTEM
HEMIC DISEASES
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
LEUKEMIA
LEUKEMIA VIRUSES
MAMMALS
MICE
MICROORGANISMS
NEOPLASMS
NUMERICAL DATA
ONCOGENIC VIRUSES
ORGANS
PARASITES
PATIENTS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
STEM CELLS
SURVIVAL CURVES
TISSUES
TRANSPLANTS
VERTEBRATES
VIRUSES
WHOLE-BODY IRRADIATION
X RADIATION