Structural investigation of adenovirus
Thesis/Dissertation
·
OSTI ID:5174684
The representation of the structural polypeptides of adenovirus has been determined using (/sup 35/S) methionine as a radiolabel for the proteins, and analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis for their separation. The known number of hexon polypeptides per virion was used to place the values on an absolute scale. Penton base was found in 60 copies, distributed as pentamers at the vertices in association with trimeric fibers. Polypeptides IX was present in 240 copies per virion, and 12 copies per group-of-nine hexon. Penton base was microheterogeneous, which could have resulted from the synthesis of penton based polypeptides from two different start codons. The relative amounts of the different penton base polypeptides suggest a mechanism by which the symmetry mismatch between penton base and fiber is resolved.
- Research Organization:
- Columbia Univ., New York (USA)
- OSTI ID:
- 5174684
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VIRUSES
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VIRUSES