Identification of the gene coding for the precursor of adenovirus core protein X

Journal Article · Sun May 01 04:00:00 EDT 1988 · Journal of Virology; (USA)

OSTI ID:6525375

Weber, J M ^[1]; Anderson, C W ^[2]

---

1. Universite de Sherbrooke, Quebec (Canada)
2. Brookhaven National Laboratory, Upton, NY (USA)

Nucleoprotein complexes extracted from infected cells or ts1 virions grown at 39{degree}C contain, in addition to known virion proteins, a polypeptide with a molecular weight of 11,000 (11K polypeptide). The authors identified the gene for this polypeptide by sequence analysis of the radioactively labeled 11K polypeptide isolated from ts1 virions and comparison of this sequence with the nucleotide sequence of the adenovirus type 2 genome. The 11K polypeptide was encoded by an open reading frame of 80 residues located between nucleotides 17,676 and 17,915 in late transcription region 2 of adenvirus type 2; the initiating methionine residue was removed leaving a 79-residue product. The late transcription region 2 that encoded the 79-residue polypeptide (11K) was arginine rich (21%) and had a predicted molecular weight of 8,715. It was cleaved by the viral endoprotease to give two products which comigrated on sodium dodecyl sulfate-polyacrylamide gels as virion polypeptide X. The data suggest that additional cleavage of the carboxy-terminal 48-residue fragment generates a 19-amino-acid fragment with the amino acid composition of mu.

• OSTI ID: 6525375
• Journal Information: Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 62:5; ISSN JOVIA; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
AMINO ACID SEQUENCE
AMINO ACIDS
ARGININE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
DRUGS
ENZYMES
EVEN-ODD NUCLEI
GENES
HYDROLASES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
MICROORGANISMS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NUCLEI
NUCLEOPROTEINS
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PEPTIDE HYDROLASES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
TRANSCRIPTION
VIRUSES