Adenovirus type 2 encoded early 11 kDa protein
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5097720
Several adenovirus type 2 (Ad2) encoded early proteins have been identified in viral infected human KB cells. These proteins are of great interest as they play key roles in cell transformation, viral DNA synthesis and gene expression. They have partially purified an AD2 encoded early polypeptide of an apparent molecular weight of 11 kilodaltons from the nuclei of viral infected cells labelled with /sup 35/S-methionine. After DNA removal from the nuclear extracts, the polypeptide was isolated using DEAE-Sephacel anion exchange and Biogel P-10 gel filtration columns. This simple two step procedure yielded several fold purification of the polypeptide. Antisera raised in mice against an Ad2 transformed rat cell line 8617 was found to immunoprecipitate the 11 kDa polypeptide from the nuclear extract of Ad2 infected KB cells. After relating this protein to an open reading frame of an Ad2 early gene block by matching the amino acid sequences to the nucleotide sequences of early genes, they plan to functionally characterize this protein by using monoclonal antibodies in in vivo and in vitro experiments.
- Research Organization:
- VA Medical Center, Allen Park, MI
- OSTI ID:
- 5097720
- Report Number(s):
- CONF-8606151-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
- Country of Publication:
- United States
- Language:
- English
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Sat Dec 31 23:00:00 EST 1983
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Thu Jul 30 00:00:00 EDT 1981
· Virology; (United States)
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OSTI ID:6162622
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL NUCLEI
CELL TRANSFORMATIONS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA REPLICATION
DRUGS
EVEN-ODD NUCLEI
GENES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
METHIONINE
MICE
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
MONOCLONAL ANTIBODIES
NUCLEI
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATHOGENESIS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
PURIFICATION
RADIOISOTOPES
RATS
RODENTS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
VIRAL DISEASES
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL NUCLEI
CELL TRANSFORMATIONS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA REPLICATION
DRUGS
EVEN-ODD NUCLEI
GENES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
METHIONINE
MICE
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
MONOCLONAL ANTIBODIES
NUCLEI
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATHOGENESIS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
PURIFICATION
RADIOISOTOPES
RATS
RODENTS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
VIRAL DISEASES