Adenovirus type 2 early proteins: assignment of the early region 1A proteins synthesized in Vivo and in Vitro to specific mRNAs

Journal Article · Thu Jul 30 04:00:00 EDT 1981 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(81)90315-9· OSTI ID:6162622

Smart, J E; Lewis, J B; Mathews, M B; Harter, M L; Anderson, C W

We have investigated the relationships among the major proteins encoded by early region 1A of Ad2 by comparative analysis of their (/sup 35/S)methionine-labeled tryptic peptides using reverse phase high-performance liquid chromatography. In some instances the purified peptides were subjected to automatic sequential Edmund degradation in order to determine the position at which the methionine residues occur within the peptides. These peptides have then been located in the amino acid sequence predicted from the DNA sequence of the leftmost 4.5% of the viral genome. The data show that the 50,000, 46,000, and 42,000 molecular weight E1A proteins (all of which focus at ca. pH 6.0) obtained from Ad2-infected HeLa cells are derived from the 1.1 kb region 1A mRNA. Similarly, it is shown that two polypeptides, 58,000 (58K) and 48,000 (48K), result from cell-free translation of the 1.1 kb mRNA. The data are also consistent with the hypothesis that the in vivo 46,000, 42,000, and 38,000 molecular weight E1A proteins (all of which focus at ca pH 5.9) are derived from the 0.9 kb region 1A mRNA. Cell-free translation of this mRNA yields two polypeptides, 54,000 (54K) and 42,000 (42K).

• Research Organization: Cold Spring Harbor Lab., NY
• OSTI ID: 6162622
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 112:2; ISSN VIRLA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
EVEN-ODD NUCLEI
GENETIC MAPPING
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
MAPPING
MESSENGER-RNA
METHIONINE
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR WEIGHT
MORPHOLOGY
MUTANTS
NUCLEI
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PH VALUE
PROTEINS
RADIOISOTOPES
RNA
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
VIRUSES