Zinc status and glutamate stimulation of calcium uptake and guinea pig cortical synaptosomes

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5173752

O'Dell, B L; Browning, J D ^[1]

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1. Univ. of Missouri, Columbia (United States)

Severe zinc deficiency adversely affects animal behavior and impairs memory. In vitro zinc is an antagonist of the NMDA receptor and Ca-channel. Since zinc deficiency impairs Ca uptake by platelets, the effect of zinc status on synaptosomal uptake of Ca, when stimulated with glutamate, was determined. Guinea pigs were fed a low Zn diet until gross pathology was evident, approximately 5 wk ({minus}Zn). Controls were fed restricted (+RF) and ad lib. (+AL). Synaptosomes were prepared from the cortex and incubated for 15 s. In a Hepes-Tris buffer that contained NA, K, Ca, EGTA, and Gly. Final K was 5 or 45, and Mg 0 or 1.3 mmol/L. Glutamate and {sup 45}Ca were added to start the reaction. When K was 45 mmol/L and Mg 0, Ca uptake was 71.6{sup a}, 118{sup b}, and 130{sup b} pmol/mg protein for the {minus}Zn, +RF and +AL groups, respectively. There was no diet effect when K was 5 mmol/L and Mg had no effect on the glutamate response. Contrary to in vitro results, zinc deficiency impairs the glutamate-stimulated calcium channel in brain.

• OSTI ID: 5173752
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY
BODY FLUIDS
BRAIN
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
GLUTAMIC ACID
GUINEA PIGS
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MATERIALS
METABOLISM
METALS
NERVE CELLS
NERVOUS SYSTEM
NUCLEI
NUTRITIONAL DEFICIENCY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES
ZINC