Does atrial natriuretic factor protect against right ventricular overload II. Tissue binding

Journal Article · Sun Oct 01 00:00:00 EDT 1989 · Journal of Applied Physiology (1985); (USA)

OSTI ID:5161667

Ou, L C; Yen, S; Sardella, G L; Hill, N S ^[1]

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1. Dartmouth Medical School, Hanover, NH (USA)

Previous studies have led us to hypothesize that the physiological significance of the diuretic and pulmonary vaso-relaxant effects of atrial natriuretic factor (ANF) is to protect the right heart. This study was designed to evaluate the relative importance of various peripheral tissues as sites of ANF action by tracing the temporal pattern of distribution of {sup 125}I-ANF and quantitating the specific binding sites. An in vivo approach, utilizing trace amount of {sup 125}I-ANF was adopted to simulate physiological conditions. {sup 125}I-ANF injected either intravenously or intra-arterially was quickly bound to peripheral tissues with less than 5% remaining in the circulation after 1 min. The relative binding capacity was greatest in the lung, followed by the kidney, right ventricle, adrenal gland, and left ventricle. The magnitude of specific ANF binding sites per gram of tissue weight followed a similar order. The data demonstrate that ANF released under all circumstances is quickly bound to the target organs, particularly the lung and the kidney, and suggest that these two organs could be the most important target organs of ANF. This evidence provides further support for the proposed hypothesis that a major evolutionary role of ANF is the protection of the right ventricle from mechanical loads.

• OSTI ID: 5161667
• Journal Information: Journal of Applied Physiology (1985); (USA), Vol. 67:4; ISSN 8750-7587
• Country of Publication: United States
• Language: English

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PEPTIDES
BIOCHEMICAL REACTION KINETICS
ADRENAL GLANDS
BIOLOGICAL FUNCTIONS
HEART
IODINE 125
KIDNEYS
LUNGS
RATS
RECEPTORS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
ANIMALS
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ENDOCRINE GLANDS
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KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
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ORGANS
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RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
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