Characterization and distribution of receptors for the atrial natriuretic peptides in mammalian brain
Both rat SVI-labeled atrial natriuretic polypeptide ( SVI-ANP or atrial natriuretic factor fragment ANF-(99-126)) and human SVI- -ANP or human ANF-(99-126)) bind with high specificity and affinity to an apparent single class of sites in guinea pig brain. Similar results have been reported in peripheral tissues, which indicate that central and peripheral ANP binding sites have fairly similar structural requirements. In vitro receptor autoradiography shows that in the guinea pig brain, SVI-ANP binding sites are highly concentrated in the external plexiform layer of the olfactory bulb, subfornical organ, various thalamic nuclei, medial geniculate nucleus, and cerebellum. Lower densities are found in the central nucleus of the amygdala, dentate gyrus, hippocampus, and area postrema. Most remaining regions contain much lower densities of sites. In rat brain SVI-ANP binding sites are differentially distributed, with high densities in the subfornical organ, area postrema, and linings of ventricles but low densities in the thalamus and cerebellum. In monkey brain, SVI-ANP binding sites are concentrated in the cerebellum. The presence of high densities of SVI-ANP binding sites in various brain regions strongly suggests the existence of a family of brain-heart peptides, in analogy to the well-known brain-gut peptides. Moreover, the extensive distribution of SVI-ANP binding sites in mammalian brain suggests that the possible roles of ANP/ANF-like peptides in brain are not restricted to the central regulation of cardiovascular parameters.
- Research Organization:
- McGill Univ., Verdun, Quebec
- OSTI ID:
- 6154122
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:1
- Country of Publication:
- United States
- Language:
- English
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