Binding and functional effects of atrial natriuretic factor in isolated rat kidney

Journal Article · Sun Nov 01 04:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:6834213

Suzuki, M; Almeida, F A; Nussenzveig, D R; Sawyer, D; Maack, T ^[1]

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1. Cornell Univ. Medical College, New York, NY (USA)

A new methodological approach was developed to study the relationship between specific binding and dose-response curves of the renal effects of atrial natriuretic factor (ANF) in isolated perfused rat kidneys (IK). IK were perfused with {sup 125}I-labeled and unlabeled ANF 1-28 to determine the following: (1) distribution, capacity (C{sub max}), and apparent affinity (S{sub 50}) of specific binding of ANF 1-28 in cortex, outer medulla, and papilla and (2) dose-response curves of the effects of ANF 1-28 on renal hemodynamics and excretion of fluid and electrolytes. The kidney had a very high density of high-affinity binding sites for ANF. Cortex had >90% of total binding sites whereas papilla had <2% of total binding sites with a 10-fold lower apparent affinity than in cortex. ANF-induced increases in glomerular filtration rate and excretion of fluid and electrolytes were detectable at 10-100 pM and maximal effects occurred at 1-10 nM ANF. Below 1 nM there was no dissociation between the renal hemodynamic and natriuretic effects of ANF. There was a close agreement between dose-response and binding curves of ANF to cortex. Results demonstrates that binding site occupancy in kidney cortex and renal effects of ANF occur at near physiological concentrations of the hormone.

• OSTI ID: 6834213
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:5; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CHEMICAL REACTIONS
CHROMATOGRAPHY
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DIURETICS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLOMERULI
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PERFUSED TISSUES
PHYSIOLOGY
POLYMERIZATION
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SEPARATION PROCESSES
TISSUE DISTRIBUTION
TISSUES
VERTEBRATES