Reduced platelet deposition on seeded versus unseeded segments of expanded polytetrafluoroethylene grafts: Clinical observations after a 6-month follow-up

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Journal of Vascular Surgery; (USA)

DOI:https://doi.org/10.1016/0741-5214(89)90410-2· OSTI ID:5158872

Ortenwall, P; Wadenvik, H; Risberg, B ^[1]

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1. Univ. of Goeteborg (Sweden)

The concept of autologous endothelial cell seeding has proved successful in animal models with respect to decrease of graft thrombogenicity and increase in patency. In the present study, application of this method in humans was explored. In 23 patients, random halves of expanded polytetrafluoroethylene grafts, used for lower limb arterial reconstructions, were seeded with endothelial cells at a seeding density of 3500 cells/cm{sup 2}. These cells were derived from the saphenous vein by enzymatic harvesting. The other half was sham seeded with culture medium. Graft thrombogenicity was estimated by measuring platelet deposition on graft surface 1 and 6 months after surgery, with indium 111-labeled platelets and external gamma-camera imaging. Seeded graft segments accumulated significantly (p less than 0.03) fewer platelets at all imaging times. It is concluded that seeding of expanded polytetrafluoroethylene vascular grafts in humans reduces graft surface thrombogenicity. The clinical implications of this remain to be demonstrated.

• OSTI ID: 5158872
• Journal Information: Journal of Vascular Surgery; (USA), Journal Name: Journal of Vascular Surgery; (USA) Vol. 10:4; ISSN 0741-5214; ISSN JVSUE
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADHESION
ANIMAL TISSUES
ARTERIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BLOOD VESSELS
BODY
BODY AREAS
BODY FLUIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
FLUORINATED ALIPHATIC HYDROCARBONS
GRAFTS
HALOGENATED ALIPHATIC HYDROCARBONS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEGS
LIMBS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC POLYMERS
ORGANS
PATIENTS
POLYETHYLENES
POLYMERS
POLYOLEFINS
POLYTETRAFLUOROETHYLENE
RADIOISOTOPE SCANNING
RADIOISOTOPES
THROMBOSIS
TISSUES
TRANSPLANTS
VASCULAR DISEASES