Influence of endothelial cell seeding on platelet deposition and patency in small-diameter Dacron arterial grafts

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Vasc. Surg.; (United States)

DOI:https://doi.org/10.1067/mva.1984.avs0010224· OSTI ID:5822490

Allen, B T; Long, J A; Clark, R E; Sicard, G A; Hopkins, K T; Welch, M J

Serial platelet deposition, surface topography, and patency were evaluated in control (N . 28) and endothelial cell-seeded (N . 28) small-diameter (4 mm inner diameter) USCI Dacron grafts implanted in the carotid and femoral arteries of dogs. All dogs received aspirin (325 mg) daily for 2 weeks starting 24 hours prior to graft implantation. Endothelial cell seeding was performed by mixing suspensions of autologous endothelial cells that had been enzymatically harvested from segments of external jugular vein with blood that was used to preclot the prostheses. The platelet deposition on each graft was quantitated by means of indium 111-labeled platelets and technetium 99m-labeled red cells in a dual-isotope platelet-imaging technique. Platelet deposition on seeded grafts 24 hours after implantation was significantly higher than on the controls (p less than 0.05). Two weeks after implantation platelet deposition on seeded prostheses had decreased to a level significantly lower than that on the controls and continued to decline on serial studies up to 7 months. In contrast to seeded grafts, platelet accumulation on control grafts dramatically increased after the withdrawal of aspirin therapy and was associated with a sharp rise in control graft thromboses. Cumulative 7-month patency for seeded prostheses was significantly higher than for the controls (96% and 29%, respectively; p less than 0.001). We conclude that endothelial cell seeding in combination with short-term aspirin therapy is a simple, reliable diameter Dacron prostheses. Abrupt withdrawal of aspirin therapy may be contraindicated in nonseeded control grafts because it results in increased platelet deposition and thrombosis.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO
• OSTI ID: 5822490
• Journal Information: J. Vasc. Surg.; (United States), Journal Name: J. Vasc. Surg.; (United States) Vol. 1:1
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETYLSALICYLIC ACID
ADHESION
ANALGESICS
ANIMALS
ANTIPYRETICS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BLOOD VESSELS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CYTOLOGICAL TECHNIQUES
DAYS LIVING RADIOISOTOPES
DISEASES
DOGS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GRAFTS
HOURS LIVING RADIOISOTOPES
HYDROXY ACIDS
IMPLANTS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEDICAL SUPPLIES
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROSTHESES
RADIOISOTOPES
SORPTIVE PROPERTIES
SURFACE PROPERTIES
TECHNETIUM 99
TECHNETIUM ISOTOPES
THROMBOSIS
TRACER TECHNIQUES
TRANSPLANTS
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES