Characteristics of estrogen-induced peroxidase in mouse uterine luminal fluid

Journal Article · Mon Apr 01 04:00:00 EST 1991 · Steroids; (United States)

DOI:https://doi.org/10.1016/0039-128X(91)90076-8· OSTI ID:5158512

Jellinck, P H; Newbold, R R; McLachlan, J A ^[1]

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1. Queen's University, Kingston, Ontario (Canada)

Peroxidase activity in the uterine luminal fluid of mice treated with diethylstilbestrol was measured by the guaiacol assay and also by the formation of 3H2O from (2-3H)estradiol. In the radiometric assay, the generation of 3H2O and 3H-labeled water-soluble products was dependent on H2O2 (25 to 100 microM), with higher concentrations being inhibitory. Tyrosine or 2,4-dichlorophenol strongly enhanced the reaction catalyzed either by the luminal fluid peroxidase or the enzyme in the CaCl2 extract of the uterus, but decreased the formation of 3H2O from (2-3H)estradiol by lactoperoxidase in the presence of H2O2 (80 microM). NADPH, ascorbate, and cytochrome c inhibited both luminal fluid and uterine tissue peroxidase activity to the same extent, while superoxide dismutase showed a marginal activating effect. Lactoferrin, a major protein component of uterine luminal fluid, was shown not to contribute to its peroxidative activity, and such an effect by prostaglandin synthase was also ruled out. However, it was not possible to exclude eosinophil peroxidase, brought to the uterus after estrogen stimulation, as being the source of peroxidase activity in uterine luminal fluid.

• OSTI ID: 5158512
• Journal Information: Steroids; (United States), Journal Name: Steroids; (United States) Vol. 56:4; ISSN 0039-128X; ISSN STEDA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
AROMATICS
ASCORBIC ACID
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
COENZYMES
CYTOCHROMES
DRUGS
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
ESTRADIOL
ESTRANES
ESTROGENS
FEMALE GENITALS
GENE REGULATION
GLUTATHIONE
HORMONES
HYDROCARBONS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
MICE
NADP
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEPTIDES
PEROXIDASES
PEROXIDES
PIGMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
POLYPEPTIDES
PROTEINS
RADIOASSAY
RADIOPROTECTIVE SUBSTANCES
RODENTS
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TYROSINE
UTERUS
VERTEBRATES
VITAMINS
WATER