Relative binding affinity of diethylstilbestrol to uterine nuclear estrogen receptor: effect of serum and serum albumin

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · Proc. Soc. Exp. Biol. Med.; (United States)

DOI:https://doi.org/10.3181/00379727-185-42573· OSTI ID:5316652

Okulicz, W C; Johnson, L D

The relative binding affinity (RBA) of tritium labelled diethylstilbestrol (DES) was determined in nuclear fractions of rat uterus. DES displayed a two- to threefold greater affinity than estradiol for nuclear E receptor. The RBA of DES to nuclear E receptor was lowered significantly in the presence of rat serum (43 +/- 1) or human serum (52 +/- 7). Dilution of human serum resulted in a progressive increase in the RBA of DES which approached that observed in the absence of serum. Addition of purified human serum albumin mimicked the decrease in RBA of DES that was observed with serum. The IC/sub 50/ of estradiol was not changed in the presence of either rat serum or albumin. These data show that DES possesses a greater affinity for nuclear E receptor than estradiol and that serum albumin can modulate DES binding to uterine E receptor.

• Research Organization: Worcester Foundation for Experimental Biology, Shrewsbury, MA (USA)
• OSTI ID: 5316652
• Journal Information: Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 185:4; ISSN PSEBA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ALBUMINS
ANIMALS
AROMATICS
BIOCHEMICAL REACTION KINETICS
BODY
ESTROGENS
FEMALE GENITALS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MAN
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PRIMATES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
STEROID HORMONES
STILBESTROL
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UTERUS
VERTEBRATES