Humoral Na sup + -K sup + pump inhibitory activity in essential hypertension and in normotensive subjects after acute volume expansion

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · American Journal of Hypertension; (USA)

OSTI ID:5157116

Pamnani, M B; Burris, J F; Jemionek, J F; Huot, S J; Price, M; Freis, E D; Haddy, F J ^[1]

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1. Uniformed Services Univ., Bethesda, MD (USA)

Plasma from black male patients with essential hypertension was bioassayed for vascular Na+-K+ pump inhibitory activity. Halves of the same rat tail artery were incubated for two hours in boiled plasma supernates from a hypertensive patient and a paired age-, sex-, and race-matched normotensive subject and then ouabain-sensitive {sup 86}Rb uptake was measured. Ouabain-sensitive {sup 86}Rb uptake by their leukocytes was also measured. Eighteen pairs of subjects were studied. The uptakes were not significantly different in the hypertensive patients and control subjects. However, when we selected from the eighteen hypertensive patients, nine with low plasma renin activity on the day of the study, uptakes were reduced in the hypertensive patients relative to the paired control subjects. We also assayed plasma supernates from normotensive black and white male subjects before and after acute volume expansion (2.5 L saline IV + 1.5 L distilled water orally over a three-hour period) and from paired normotensive subjects before and after sham volume expansion and obtained a positive bioassay in the expanded subjects both on intraindividual and interindividual comparisons. These studies demonstrate increased vascular Na+-K+ pump inhibitory activity in the plasma of black male patients with low renin essential hypertension and in the plasma of normotensive subjects after acute volume expansion. The findings suggest that the inhibitory activity in the hypertensive subjects' plasma is related to volume expansion, relative or absolute.

• OSTI ID: 5157116
• Journal Information: American Journal of Hypertension; (USA), Vol. 2:7; ISSN 0895-7061
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HYPERTENSION
PATHOGENESIS
POTASSIUM COMPOUNDS
MEMBRANE TRANSPORT
SODIUM COMPOUNDS
INHIBITION
LEUKOCYTES
MINORITY GROUPS
PEPTIDES
RATS
RENIN
RUBIDIUM 86
TRACER TECHNIQUES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARDIOVASCULAR DISEASES
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
HUMAN POPULATIONS
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
POPULATIONS
PROTEINS
RADIOISOTOPES
RODENTS
RUBIDIUM ISOTOPES
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES
550901* - Pathology- Tracer Techniques