Proton translocation coupled to dimethyl sulfoxide reduction in anaerobically grown Escherichia coli HB101
Proton translocation coupled to dimethyl sulfoxide (DMSO) reduction was examined in Escherichia coli HB101 grown anaerobically on glycerol and DMSO. Rapid acidification of the medium was observed when an anaerobic suspension of cells, preincubated with glycerol, was pulsed with DMSO, methionine sulfoxide, nitrate, or trimethylamine N-oxide. The DMSO-induced acidification was sensitive to the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (60 microM) and was inhibited by the quinone analog 2-n-heptyl-4-hydroxy-quinoline-N-oxide (5.6 microM). Neither sodium azide nor potassium cyanide inhibited the DMSO response. An apparent----H+/2e- ratio of 2.9 was obtained for DMSO reduction with glycerol as the reductant. Formate and H2(g), but not lactate, could serve as alternate electron donors for DMSO reduction. Cells grown anaerobically on glycerol and fumarate displayed a similar response to pulses of DMSO, methionine sulfoxide, nitrate, and trimethylamine N-oxide with either glycerol or H2(g) as the electron donor. However, fumarate pulses did not result in acidification of the suspension medium. Proton translocation coupled to DMSO reduction was also demonstrated in membrane vesicles by fluorescence quenching. The addition of DMSO to hydrogen-saturated everted membrane vesicles resulted in a carbonyl cyanide p-trifluoromethoxyphenyl-hydrazone-sensitive fluorescence quenching of quinacrine dihydrochloride. The data indicate that reduction of DMSO by E. coli is catalyzed by an anaerobic electron transport chain, resulting in the formation of a proton motive force.
- Research Organization:
- Univ. of Alberta, Edmonton, Canada
- OSTI ID:
- 5148338
- Journal Information:
- J. Bacteriol.; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CULTURE MEDIA
ACIDIFICATION
DMSO
METABOLISM
ESCHERICHIA COLI
GROWTH
PROTONS
TRANSLOCATION
ANAEROBIC CONDITIONS
AZIDES
BIOCHEMICAL REACTION KINETICS
CYANIDES
FORMATES
GLYCEROL
METHIONINE
NITRATES
POTASSIUM
QUINOLINES
REDOX REACTIONS
SODIUM
SULFOXIDES
ALCOHOLS
ALKALI METALS
AMINO ACIDS
AROMATICS
AZAARENES
AZINES
BACTERIA
BARYONS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DRUGS
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
LIPOTROPIC FACTORS
METALS
MICROORGANISMS
NITROGEN COMPOUNDS
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PYRIDINES
REACTION KINETICS
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)