Sugar production from agricultural woody wastes by saccharification with Trichoderma viride cellulase

Conference · Wed Jan 01 04:00:00 EST 1975 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:5142482

Toyama, N; Ogawa, K

The saccharification of agricultural woody wastes was studied using a commercial enzyme preparation, Cellulase onozuka, derived from Trichoderma viride or the solid culture extracts of the fungus. With the intention of producing sugar at low cost, a simple procedure of enzymatic saccharification of rice straw, bagasse, and sawdust was studied. Delignifying methods of these wastes were investigated using dilute sodium hydroxide solution and dilute peracetic acid. Rice straw and bagasse were effectively delignified by boiling in a 1 percent sodium hydroxide solution for 3 hr or by autoclaving at 120/sup 0/C in a 1 percent sodium hydroxide solution for 1 hr. The sawdust from a broad leaved tree (Machilus thunbergii) was delignified by autoclaving at 120/sup 0/C in a 1 percent sodium hydroxide solution for 1 hr and by subsequent boiling in diluted /sup 1///sub 5/ peracetic acid for 1 hr. This type of sawdust was also delignified by boiling in /sup 1///sub 5/ peracetic acid for 1 hr and by subsequent autoclaving at 120/sup 0/C in a 1 percent sodium hydroxide solution for 1 hr. The sawdust from a coniferous tree (Cryptomeria japonica) was delignified by boiling in /sup 1///sub 5/ peracetic acid for 1 hr and by subsequent autoclaving at 120/sup 0/C in a 1 percent sodium hydroxide solution for 1 hr; however, the successive treatment by autoclaving with alkali solution and subsequent boiling with diluted peracetic acid failed to bring about the desired effect. The saccharification of delignified rice straw, bagasse, and sawdust was examined using Cellulase onozuka, wheat bran or rice straw solid culture at various substrate concentrations, resulting in the formation of 5 to 10 percent sugar solutions after incubation at pH 5.0, 45/sup 0/C for 48 hr. The optimum substrate concentration existed at around 10 percent. Reuse of cellulase solution and resaccharification of residual sawdust were considered to be inadequate.

• Research Organization: Miyazaki Univ., Japan
• OSTI ID: 5142482
• Conference Information: Journal Name: Biotechnol. Bioeng. Symp.; (United States) Journal Volume: 5
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
AGRICULTURAL WASTES
BAGASSE
BIOMASS
BIOSYNTHESIS
CARBOHYDRATES
CELLULASES
CHEMICAL REACTIONS
DECOMPOSITION
DELIGNIFICATION
ENERGY SOURCES
ENZYMATIC HYDROLYSIS
ENZYMES
HYDROLASES
HYDROLYSIS
LYSIS
ORGANIC COMPOUNDS
RENEWABLE ENERGY SOURCES
SACCHARIDES
SOLID WASTES
SOLVOLYSIS
SYNTHESIS
WASTES
WOOD
WOOD WASTES