Feasibility of sugar production from agricultural and urban cellulosic wastes with Trichoderma viride cellulase

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:5134812

Toyama, N

Our present studies on enzymatic saccharification of various cellulosic wastes are concentrated on the production of thick sugar solutions. Recent Trichoderma viride cellulase preparations produced by wheat bran solid cultures reached the highest strength. Almost complete saccharification of delignified rice straw and bagasse and the production of thick sugar solution from these substrates have been achieved by using a 1 percent solution of an active T. viride commercial preparation, Cellulase onozuka CLA 762, after incubation at 10 and 20 percent substrate concentrations, pH 5.0, 45/sup 0/C for 48 hr. From an economical viewpoint, the production of T. viride cellulase must be developed using cellulosic wastes, and suitable nitrogen sources for cellulase production should also be selected from among agricultural wastes. The saccharification of delignified rice straw and bagasse was undertaken by using the rice straw solid culture of the fungus or the solid culture extract. An important factor affecting economical sugar production is the choice of an adequate delignifying method for each cellulosic waste. Lignin in cellulosic wastes markedly influences the cellulase production by T. viride in solid culture. An attempt to produce sugar solutions was carried out by autosaccharifying solid cultures inoculated with T. viride in the presence of water.

• Research Organization: Miyazaki Univ., Japan
• OSTI ID: 5134812
• Journal Information: Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 6; ISSN BIBSB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AGRICULTURAL WASTES
ALDEHYDES
BIOMASS
BIOSYNTHESIS
CARBOHYDRATES
CELLULOSE
CHEMICAL REACTIONS
CULTURE MEDIA
DECOMPOSITION
ENERGY SOURCES
ENZYMATIC HYDROLYSIS
FEASIBILITY STUDIES
FUNGI
GLUCOSE
HEXOSES
HYDROLYSIS
LYSIS
MONOSACCHARIDES
MUNICIPAL WASTES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
RENEWABLE ENERGY SOURCES
SACCHARIDES
SOLID WASTES
SOLVOLYSIS
SYNTHESIS
TRICHODERMA
TRICHODERMA VIRIDE
WASTES