Ethanol production via fungal decomposition and fermentation of biomass. Semiannual progress report, October 1981-March 1982

Technical Report · Thu Apr 01 04:00:00 EST 1982

OSTI ID:5140585

Antonopoulos, A A; Wene, E G

During the first half of FY 1982, a few additional Fusarium isolates were obtained and screened for their cellulolytic and fermenting abilities. Experiments with an isolate from Fusarium strain ANL 22-760 showed that cellulose was the best inducer of FPase and CMCase, while glucose, lactose, and xylose almost completely inhibited cellulase production. Tests with Fusarium strain ANL 11182 indicated that a cellulase production of 2.5 IU/mL in 14 days was obtained at 28/sup 0/C in a medium of 1% cellulose and in the presence of 0.7 mg/mL extracellular protein. Increasing the nitrogen content of the medium stimulated cell growth but decreased cellulase production. The ratio of CMCase to FPase remained nearly constant at about 10:1 during fermentations with varying concentrations of cellulose and nitrogen sources. Several of the new isolates and mutants, when screened for their fermenting ability in glucose, produced ethanol at a conversion efficiency close to that achieved in yeast fermentations. Fermentations of 1% glucose by isolates from strain ANL 99A-78 repeatedly produced 4.3 mg/mL ehtanol within 48 hours. A few of the new isolates and mutants produced, in consecutive fermentations of 1% xylose, up to 4.2 mg/mL ethanol within 48 hours. Addition of glucose to higher concentrations of xylose increased the yield of ethanol.

• Research Organization: Argonne National Lab., IL (USA)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5140585
• Report Number(s): ANL/EES-TM-187; ON: DE82020401
• Country of Publication: United States
• Language: English

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ALCOHOLS
ALDEHYDES
BIOCONVERSION
BIOMASS
BIOSYNTHESIS
CARBOHYDRATES
CELLULASE
CELLULOSE
CHEMICAL REACTIONS
CULTURE MEDIA
DECOMPOSITION
DISACCHARIDES
ENERGY SOURCES
ENZYMES
ETHANOL
FERMENTATION
FUNGI
FUSARIUM
GLUCOSE
GLYCOSYL HYDROLASES
GROWTH
HEXOSES
HYDROLASES
HYDROXY COMPOUNDS
LACTOSE
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PARASITES
PENTOSES
PLANT GROWTH
PLANTS
POLYSACCHARIDES
RENEWABLE ENERGY SOURCES
SACCHARIDES
SYNTHESIS
XYLOSE