Ethanol production via fungal decomposition and fermentation of biomass. Phase II (FY 1981) annual progress report

Technical Report · Thu Oct 01 04:00:00 UTC 1981

DOI:https://doi.org/10.2172/5565773· OSTI ID:5565773

Antonopoulos, A. A.; Wene, E. G.

This program has as its main goal the isolation and development of Fusarium strains that can efficiently and economically decompose plant polysaccharides to pentoses and hexoses and ferment them to ethanol for fuel purposes. During Phase II (FY 1981) of this program, more than 800 new Fusarium isolates were isolated and screened. All showed cellulolytic activity. The Fusarium mutant ANL 3-72181 (derived after uv exposure of ANL 22 isolate) produced 2.45 iu cellulase after 14 days. This cellulase activity was achieved in the presence of 0.7 mg/mL extracellular protein. In separate tests, the use of both proteose peptone and yeast extract with 1% cellulose increased the production of extracellular protein three times over that on cellulose alone. Initial fermentation by Fusarium strains on 1% glucose produced up to 4.2 mg/mL ethanol in 48 hours. All Fusarium isolates and mutants found during this period were screened for xylose fermentation. Ethanol production during early experimentation required from 120 to 144 hours to yield 4.0 to 4.5 mg/mL ethanol from 1% xylose solutions. Through continuous selection of isolates, this time was reduced to 66 hours. By recycling Fusarium cell mass, fermentations of 1% xylose yielded 4.0 to 4.3 mg/mL ethanol in 48 hours. Consecutive fermentations of 2% xylose produced an average of 8.1 mg/mL ethanol in 48 hours. Fermentation of a 4.5% xylose + 2% glucose solution produced 21 mg/mL ethanol and 0.8 mg/mL acetic acid, while fermentation of a 7% xylose + 2% glucose solution yielded 25.5 mg/mL ethanol and 0.85 mg/mL acetic acid; these fermentations were aerated at a rate of 0.03 v/v-min.

• Research Organization: Argonne National Lab., IL (USA)
• Sponsoring Organization: USDOE
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5565773
• Report Number(s): ANL/EES-TM-162; ON: DE82009492
• Country of Publication: United States
• Language: English

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ACETIC ACID
AERATION
ALCOHOLS
ALDEHYDES
BIOCONVERSION
BIOMASS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELLULASE
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENERGY SOURCES
ENZYMES
ETHANOL
FERMENTATION
FUNGI
FUSARIUM
GLUCOSE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROXY COMPOUNDS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PENTOSES
PLANTS
POLYSACCHARIDES
PRODUCTION
RENEWABLE ENERGY SOURCES
SACCHARIDES
XYLOSE
YIELDS