Physico-chemical methods for the study of polycyclic aromatic hydrocarbon carcinogen-DNA interactions: Progress report, 3/1/85 to 2/29/88

Technical Report · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5130339

The goal of this project was to develop new spectroscopic techniques and to adapt existing methods for studies of the interactions of polycyclic aromatic mutagens and carcinogens with nucleic acids. A highly sensitive, computer-interfaced linear dichroism system was constructed to allow for studies of the conformations of carcinogen-DNA non-covalent complexes and covalent adducts. Fluorescence techniques were adapted for studies of the heterogeneities of carcinogen-DNA adducts utilizing fluorescence emission spectra, and detailed comparisons between excitation and absorption spectra acquired with digital instrumentation. Detailed fluorescence quenching studies, together with accurate determinations of fluorescence decay profiles utilizing the National Synchrotron Light Source at the Brookhaven National Laboratory, allowed for a determination of the solvent exposure of the polycyclic aromatic moieties in covalent carcinogen-DNA adducts. Finally, a detailed study of the base-sequence dependence of non-covalent intercalation complex formation and chemical reactivity of poycyclic aromatic diol epoxide with synthetic polynucleotides was performed. These studies provided insight into the detailed mechanisms of interaction of benzo(a)pyrene trans-7,8-dihydrodiol-9,10-epoxide with DNA. 19 refs., 4 figs., 1 tab.

• Research Organization: New York Univ., NY (USA). Dept. of Chemistry
• DOE Contract Number: AC02-78EV04959
• OSTI ID: 5130339
• Report Number(s): DOE/EV/04959-10; ON: DE88009247
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
DNA ADDUCTS
DICHROISM
OLIGONUCLEOTIDES
BENZOPYRENE
CHEMICAL REACTION KINETICS
PROGRESS REPORT
ADDUCTS
AROMATICS
CONDENSED AROMATICS
DOCUMENT TYPES
HYDROCARBONS
KINETICS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry