Characterization of the microdialysis junction interface for capillary electrophoresis/microelectrospray ionization mass spectrometry

Journal Article · Sun Jun 01 04:00:00 EDT 1997 · Analytical Chemistry (Washington)

DOI:https://doi.org/10.1021/ac9611226· OSTI ID:512892

Severs, J C; Smith, R D ^[1]

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1. Pacific Northwest National Lab., Richland, WA (United States)

A capillary electrophoresis/electrospray ionization mass spectrometry (CE/ESI-MS) interface, based on an electric circuit across a microdialysis membrane surrounding a short capillary segment closely connected to the separation capillary terminus, is demonstrated to be sensitive, efficient, and rugged. A microspray type ionization emitter produces a stable electrospray at the low flow rates provided by CE and thus avoids both the need for a makeup liquid flow provided by liquid junction or sheath flow interfaces and the subsequent dilution and reduction in sensitivity. Reproducibility studies and comparisons with CE/UV and the CE/sheath flow interface with ESI-MS are presented. Additionally, postrun acidification via the microdialysis junction interface is demonstrated and shown to be capable of denaturing the holomyoglobin protein noncovalent complex while maintaining separation efficiency. 21 refs., 7 figs., 1 tab.

• DOE Contract Number: AC06-76RL01830
• OSTI ID: 512892
• Journal Information: Analytical Chemistry (Washington), Journal Name: Analytical Chemistry (Washington) Journal Issue: 11 Vol. 69; ISSN ANCHAM; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
42 ENGINEERING
55 BIOLOGY AND MEDICINE
BASIC STUDIES
CAPILLARIES
DESIGN
DIALYSIS
ELECTROPHORESIS
INTERFACES
IONIZATION
MASS SPECTROMETERS
MEMBRANES
MICROELECTRONICS
MYOGLOBIN
NUMERICAL DATA
ON-LINE SYSTEMS
PROTEINS
SEPARATION PROCESSES
SPRAYS