Characterization of the microdialysis junction interface for capillary electrophoresis/microelectrospray ionization mass spectrometry
- Pacific Northwest National Lab., Richland, WA (United States)
A capillary electrophoresis/electrospray ionization mass spectrometry (CE/ESI-MS) interface, based on an electric circuit across a microdialysis membrane surrounding a short capillary segment closely connected to the separation capillary terminus, is demonstrated to be sensitive, efficient, and rugged. A microspray type ionization emitter produces a stable electrospray at the low flow rates provided by CE and thus avoids both the need for a makeup liquid flow provided by liquid junction or sheath flow interfaces and the subsequent dilution and reduction in sensitivity. Reproducibility studies and comparisons with CE/UV and the CE/sheath flow interface with ESI-MS are presented. Additionally, postrun acidification via the microdialysis junction interface is demonstrated and shown to be capable of denaturing the holomyoglobin protein noncovalent complex while maintaining separation efficiency. 21 refs., 7 figs., 1 tab.
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 512892
- Journal Information:
- Analytical Chemistry (Washington), Vol. 69, Issue 11; Other Information: PBD: 1 Jun 1997
- Country of Publication:
- United States
- Language:
- English
Similar Records
Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry
Characterization of Microdialysis Acidification for Capillary Isoelectric Focusing Microelectrospray Ionization Mass Spectrometry