Organization of genes necessary for growth of the hydrogen methanol autotroph Xanthobacter sp. strain H4-14 on hydrogen and carbon dioxide
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:5127684
Mutants unable to grow on H/sub 2/ and CO/sub 2/ were isolated in the hydrogen-methanol autotroph Xanthobacter sp. strain H4-14 and complemented with a clone bank constructed in a broad-host-range cosmid vector. The mutants fell into two classes. Class 1 mutants (Cfx/sup -/) cannot grow on hydrogen or methanol and are deficient in one or more of the key enzymes of the Calvin Cycle. Class 2 mutants (Hox/sup -/) can grow on methanol but not on hydrogen and lack hydrogenase activity. Restriction maps of the complementing clones show that each class is not linked to the other. Subcloning and Tn5 mutagenesis have localized the regions of DNA complementing these mutants. The region complementing a class 1 mutant which is deficient in ribulosebisphosphate carboxylase activity is approximately 3.2 kilobase pairs in size. Expression of this enzyme activity from cloned DNA gave evidence for the orientation of an operon containing the structural genes for this enzyme. The region complementing most of the class 2 mutants is 3 to 4.5 kilobase pairs in size.
- Research Organization:
- Univ. of Washington, Seattle
- OSTI ID:
- 5127684
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 162:3; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
550400 -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGICAL PATHWAYS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CHALCOGENIDES
CLONING
DNA-CLONING
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GENES
GENETIC MAPPING
GROWTH
HYDROGEN
LYASES
MAPPING
MICROORGANISMS
MUTAGENESIS
MUTANTS
NONMETALS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
TRANSFERASES
550400 -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGICAL PATHWAYS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CHALCOGENIDES
CLONING
DNA-CLONING
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GENES
GENETIC MAPPING
GROWTH
HYDROGEN
LYASES
MAPPING
MICROORGANISMS
MUTAGENESIS
MUTANTS
NONMETALS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
TRANSFERASES