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Molecular cloning of a malyl coenzyme A lyase gene from Pseudomonas sp. strain AM1, a facultative methylotroph

Journal Article · · J. Bacteriol.; (United States)
OSTI ID:5995986
; ;
A genomic library containing HindIII partial digest of Pseudomonas sp. strain AM1 DNA was constructed in the broad-host-range cosmid pVK100. PCT57, a Pseudomonas sp. strain AM1 methanol mutant deficient in malyl coenzyme A lyase activity, was complemented to a methanol-positive phenotype by mobilization of pVK100 library into PCT57 recipients with the ColE1/RK2 mobilizing plasmid pRK2013. Six different complemented isolates all contained a recombinant plasmid carrying the same 19.6-kilobase-pair Pseudomonas sp. strain AM1 DNA insert. Subcloning and complementation analysis demonstrated that the gene deficient in PCT57 (mcl-1) was located in a 1.6-kilobase-pair region within a 7.4-kilobase-pair EcoRI-HindIII fragment. 33 references, 3 figures, 3 tables.
Research Organization:
Univ. of Washington, Seattle
DOE Contract Number:
AT06-80ER10680
OSTI ID:
5995986
Journal Information:
J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 160:2; ISSN JOBAA
Country of Publication:
United States
Language:
English

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Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGY
CLONING
DATA
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
GENES
GENETIC EFFECTS
GENETICS
GENOME MUTATIONS
HYDROXY COMPOUNDS
INFORMATION
LYASES
METHANOL
MICROORGANISMS
MUTANTS
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
PHENOTYPE
PSEUDOMONAS