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Genetic locus essential for formate-dependent growth of Bradyrhizobium japonicum. [Ribulosebisphosphate carboxylase; Phosphoribulokinase]

Journal Article · · J. Bacteriol.; (United States)
OSTI ID:6077015
;
A genetic locus essential for the formate-dependent growth of Bradyrhizobium japonicum was isolated by complementation of ethyl methanesulfonate-induced mutants with a cosmid gene library of B. japonicum DNA. Three related cosmids containing 18.7 kilobase pairs of B. japonicum DNA in common were identified as being able to restore formate-dependent growth capability to mutants lacking either ribulosebisphosphate carboxylase or both ribulosebisphosphate carboxylase and phosphoribulokinase activities. To further localize the complementing gene(s), a series of four deletions spanning a total of 16.1 kilobase pairs were introduced into the B. japonicum chromosome. Each resulting deletion mutant lacked formate dehydrogenase activity and lacked ribulosebisphosphate carboxylase activity and immunologically detectable protein. Three of the four also lacked phosphoribulokinase activity. Two other mutants in which the deletion-bearing recombinant plasmid had integrated into the chromosome also lacked ribulosebisphosphate carboxylase activity and protein and phosphoribulokinase activities. The genetic locus defined by these mutants could contain the structural genes for these enzymes or a regulatory gene(s) controlling their expression or both.
Research Organization:
Michigan State Univ., East Lansing
DOE Contract Number:
AC02-76ER01338
OSTI ID:
6077015
Journal Information:
J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 169:7; ISSN JOBAA
Country of Publication:
United States
Language:
English

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Related Subjects

550400* -- Genetics
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CARBOXYLIC ACID SALTS
EMS
ENZYMES
ESTERS
FORMATES
GENETIC MAPPING
LYASES
MAPPING
MICROORGANISMS
MOLECULAR BIOLOGY
MUTAGENESIS
MUTAGENS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RHIZOBIUM
SULFONIC ACID ESTERS
TRANSFERASES