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Effects of propionate and methylmalonate on conversions of acetate, butyrate, and D(-)-3-hydroxybutyrate to fatty acids and carbon dioxide by mammary tissue slices of goats

Journal Article · · J. Dairy Sci.; (United States)
Incorporations of (1-carbon-14) acetate, (1-carbon-14) propionate, n-(1-carbon-14) butyrate, and D(-)-3-hydroxy(3-carbon-14) butyrate into individual milk fatty acids and their conversion to carbon dioxide were studied in vitro with caprine mammary tissue slices in the presence and absence of propionate and methylmalonate. Neither propionate nor methylmalonate affected incorporation of these substances into fatty acids. In a decreasing order butyrate, acetate, propionate, and D(-)-3-hydroxybutyrate were converted to carbon dioxide. Acetate had the highest incorporation rate into fatty acids followed by D(-)-3-hydroxybutyrate, butyrate, and propionate. Labeled propionate was incorporated mainly into odd-numbered fatty acids. Results do not support the theory that either propionate or its metabolite, methylmalonate, inhibit de novo synthesis of fatty acids in the mammary gland in relation to the etiology of low milk fat syndrome.
Research Organization:
Univ. of Alberta, Edmonton, Canada
OSTI ID:
5125332
Journal Information:
J. Dairy Sci.; (United States), Journal Name: J. Dairy Sci.; (United States) Vol. 3; ISSN JDSCA
Country of Publication:
United States
Language:
English