Effects of volatile fatty acids on propionate metabolism and gluconeogenesis in caprine hepatocytes
Isolated caprine hepatocytes were incubated with fatty acids of various chain lengths. Short-chain fatty acids effects on rates of gluconeogenesis and oxidation from (2-/sup 14/C) propionate were determined. Additions of glucose (2.5 mM) had no effect on hepatic (2-/sup 14/C)-propionate metabolism in the presence and absence of amino acids. A complete mixture of amino acids increased label incorporation from (2-/sup 14/C) propionate into (/sup 14/C) glucose by 22%. Butyrate inhibited (2-/sup 14/C) propionate metabolism and increased the apparent Michaelis constant for (2-/sup 14/C) propionate incorporation into (/sup 14/C) glucose from 2.4 +/- 1.5 to 5.6 +/- .9 mM. Butyrate's effects on propionate were similar in the presence and absence of L-carnitine (1 mM). Isobutyrate, 2-methylbutyrate, and valerate (1.25 mM) had no effect on (/sup 14/C) glucose production but decreased /sup 14/CO/sub 2/ production to 57, 61, and 54% of the control (2-/sup 14/C) propionate (1.25 mM). This inhibition on /sup 14/CO/sub 2/ was not competitive. Isovalerate had no effect on either (2-/sup 14/C) propionate incorporation into glucose of CO/sub 2/. An increase in ratio of (/sup 14/C) glucose to /sup 14/CO/sub 2/ from (2-/sup 14/C)-propionate demonstrated that short-chain fatty acids other than butyrate do not inhibit gluconeogenesis from propionate. In addition, fatty acids that generate a net synthesis of intracellular oxaloacetate may partition propionate carbons toward gluconeogenic rather than oxidative pathways in goat hepatocytes.
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 5242831
- Journal Information:
- J. Dairy Sci.; (United States), Journal Name: J. Dairy Sci.; (United States) Vol. 70:12; ISSN JDSCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
553005 -- Agriculture & Food Technology-- Animal Husbandry-- (1987-)
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ALDEHYDES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DOMESTIC ANIMALS
GLUCOSE
GOATS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER CELLS
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROPIONIC ACID
RUMINANTS
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES