Experimental myocardial infarct imaging following intravenous administration of iodine-131 labeled antibody (Fab')/sub 2/ fragments specific for cardiac myosin. [/sup 141/Ce scintiscanning]
Canine myocardial infarcts resulting from ligation of the left anterior descending coronary artery were localized in vivo by gamma scintigraphy following the intravenous injection of /sup 131/I anticardiac myosin antibody (Fab')/sub 2/. The anteroapical location of the image was confirmed by demonstration of the blood pool with /sup 99m/Tc sulfur colloid, and by subsequent imaging of the excised heart. The scintigram of the excised heart, following prior in vivo injection of /sup 141/Ce-microspheres, showed a region of diminished radioactivity near the apex which corresponded precisely to the region of /sup 131/I antibody (Fab')/sub 2/ uptake. Well defined areas of /sup 131/I antibody (Fab')/sub 2/ activity in the region of infarction were consistently imaged at 48 hours in animals in which the ligature occluding the coronary artery was released at 5 hours; 72 hours were at times required when coronary occlusion persisted throughout the experiment. In both reflow and persistent occlusion models, the concentration of /sup 131/I antibody (Fab')/sub 2/ was inversely related to blood flow as determined from microsphere distribution in the region of infarction; though in areas of equivalent flow (0 to 20% of normal) the mean concentration ratio of antibody uptake to normal tissue was 20.7 +- 2.2 in the reflow model as compared to 11.4 +- 0.7 in the persistent occlusion model. A reduction in blood flow to the affected area of <50% of normal did not result in the production of a localized scintigraphic image.
- Research Organization:
- Massachusetts General Hospital, Boston
- OSTI ID:
- 5113265
- Journal Information:
- Circulation; (United States), Journal Name: Circulation; (United States) Vol. 57:4; ISSN CIRCA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD FLOW
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CERIUM 141
CERIUM ISOTOPES
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DOGS
EVEN-ODD NUCLEI
GLOBULINS
HEART
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOTOPES
MAMMALS
MICROSPHERES
MYOCARDIAL INFARCTION
MYOSIN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RARE EARTH ISOTOPES
RARE EARTH NUCLEI
SCINTISCANNING
VERTEBRATES