Reversal of impaired wound healing in irradiated rats by platelet-derived growth factor-BB

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · American Journal of Surgery; (USA)

DOI:https://doi.org/10.1016/0002-9610(89)90131-1· OSTI ID:5099178

Mustoe, T A; Purdy, J; Gramates, P; Deuel, T F; Thomason, A; Pierce, G F ^[1]

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1. Washington Univ. Medical Center, St. Louis, MO (USA)

This study examined the potential influence of platelet-derived growth factor-BB homodimers (PDGF-BB) on surgical incisions in irradiated animals with depressed wound healing. Rats were irradiated with either 800 rads total body or 2,500 rads surface irradiation. Parallel dorsal skin incisions were made 2 days later, and PDGF-BB was applied topically a single time to one of two incisions. In total body-irradiated rats, bone marrow-derived elements were severely depressed, wound macrophages were virtually eliminated, and PDGF-BB treatment was ineffective. However, in surface-irradiated rats, PDGF-BB treatment recruited macrophages into wounds and partially reversed impaired healing on day 7 (p less than 0.005) and day 12 (p less than 0.001). PDGF-BB-treated wounds were 50 percent stronger than the paired control wounds. The results suggest PDGF requires bone marrow-derived cells, likely wound macrophages, for activity and that it may be useful as a topical agent in postirradiation surgical incisions.

• OSTI ID: 5099178
• Journal Information: American Journal of Surgery; (USA), Journal Name: American Journal of Surgery; (USA) Vol. 158:4; ISSN 0002-9610; ISSN AJSUA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL FUNCTIONS
BIOLOGICAL RECOVERY
BODY
BONE MARROW
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
EXTERNAL IRRADIATION
FIBROBLASTS
FUNCTIONS
GROWTH FACTORS
HEALING
HEMATOPOIETIC SYSTEM
INJURIES
IRRADIATION
MACROPHAGES
MAMMALS
MEDICINE
MITOGENS
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PROTEINS
RADIOLOGY
RADIOTHERAPY
RATS
RECOVERY
RODENTS
SKIN
SOMATIC CELLS
THERAPY
TISSUES
VERTEBRATES
WHOLE-BODY IRRADIATION
WOUNDS