Altered mitochondrial function after acute alteration of the endogenous insulin/glucagon ratio
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5097598
Mannoheptulose (MH) affects pancreatic Islet cells to cause a drop in serum insulin and a rise in glucagon. This effect peaks 1 hr after injection and results in a 3-fold increase in serum glucose. Here they examined whether metabolic functions of liver mitochondria (mito) are altered by this change in hormone status. Rats fed ad lib on 12 hr light/dark cycles were given MH (2g/kg) or vehicle i.p. during the first 2 hrs of the light cycle. Liver mito were isolated 1 hr later. Acid-extracts were assayed for ATP+ADP+AMP (nmol/mg prot). Citrulline synthesis and pyruvate carboxylation rates (nmol/min/mg prot) were assayed by following H(/sup 14/C)O/sub 3//sup -/ fixation in appropriate media. State 3 and 2,4-DNP-uncoupled respiratory rates (1/2 nmol O/sub 2//min/mg prot) were assayed polarographically with succinate. The effects of MH on mito are comparable to reported effects of glucagon injection. MH evokes acute reciprocal changes in insulin and glucagon that are highly reproducible. Thus, MH offers an interesting model for studying the effect of endogenous hormones on mito functions.
- Research Organization:
- Tufts Univ., Medford, MA
- OSTI ID:
- 5097598
- Report Number(s):
- CONF-8606151-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
ALDEHYDES
AMP
ANIMALS
ATP
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
GLANDS
GLUCAGON
HEXOSES
HORMONES
INSULIN
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
MANNOSE
MITOCHONDRIA
MONOSACCHARIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PANCREAS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
QUANTITY RATIO
RATS
RODENTS
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADP
ALDEHYDES
AMP
ANIMALS
ATP
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
GLANDS
GLUCAGON
HEXOSES
HORMONES
INSULIN
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
MANNOSE
MITOCHONDRIA
MONOSACCHARIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PANCREAS
PEPTIDE HORMONES
PEPTIDES
POLYPEPTIDES
PROTEINS
QUANTITY RATIO
RATS
RODENTS
SACCHARIDES
TRACER TECHNIQUES
VERTEBRATES