Induction of N-glucosylation in activated B-lymphocytes

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5096947

Rush, J S; Summers, L; Snow, C E; Waechter, C J

Proliferation of resting B-lymphocytes (B-cells) can be induced by a variety of mitogens. To investigate changes in membrane glycoprotein synthesis that might accompany activation, B-cells have been isolated from the spleens of female DBA/2 mice, and the preparations depleted of T-lymphocytes by incubation with monoclonal anti-Thy 1 antibody and complement. N-Glycosylation rates were assessed by following the incorporation of (/sup 3/H)mannose into dolichol-linked oligosaccharides and glycoprotein before and after exposure to bacterial lipopolysaccharide (LPS) from S. typhosa. Activation of B-cells with LPS resulted in a 6-10 fold increase in the incorporation of radiolabeled mannose into oligosaccharide lipid intermediates and glycoprotein. Labeling of dolichol-linked oligosaccharides reaches a maximum at 20 hr, preceding a peak of glycoprotein labeling at 24-36 hr. N-glycosylation activity is maximal at LPS concentrations of 5-50 ..mu..g/ml. Labeling experiments with (/sup 3/H)mannose, (/sup 3/H)uridine and (/sup 3/H)thymidine, indicate that n-glycosylation activity is induced during the transition of the resting B-cell from G/sub 0/ to G/sub 1/, and does not require that the cells enter S-phase.

• Research Organization: Univ. of Kentucky College of Medicine, Lexington
• OSTI ID: 5096947
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
ANTIBODIES
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CELL PROLIFERATION
COMPLEMENT
CONNECTIVE TISSUE CELLS
GLUCOPROTEINS
HETEROCYCLIC COMPOUNDS
HEXOSES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LABELLING
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOCYTES
MANNOSE
MATERIALS
MEMBRANES
MONOCLONAL ANTIBODIES
MONOSACCHARIDES
NUCLEOSIDES
NUCLEOTIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
URIDINE