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Induction of N-glycosylation activity in B-cells by PMA and ionomycin

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6156634
; ;
Although N-glycosylation activity in murine splenic B-lymphocytes (B-cells) was not stimulated by phorbol 12-myristate 13-acetate (PMA) or ionomycin added separately, the combination of PMA plus ionomycin produced a dramatic increase in the incorporation of (TH)mannose into dolichol-linked oligosaccharides and glycoprotein. Maximal stimulation of N-glycosylation activity was observed after exposure of B-cells to 1 M ionomycin in the presence of 1-10 ng/ml PMA for 36 hr. At these concentrations of PMA and ionomycin, RNA and DNA synthesis, which required 1% and 5% fetal calf serum, respectively, peaked at 20-24 hr. 1-(5-Isoquinolinylsulfonyl)-2-methylpiperazine (H-7), a potent inhibitor of protein kinase C, prevented the stimulation of N-glycosylation activity, RNA synthesis and DNA synthesis. N-(2-(Methylamino)ethyl)-5-isoquinolinesulfonamide (H-8), an effective inhibitor of cyclic nucleotide-dependent protein kinases, reduced N-glycosylation activity, but required a higher concentration than H-7. These results support the conclusion that protein kinase C plays a key role in the activation of B-cells. Current work is aimed at determining if any enzymes in the dolichol pathway are activated directly by protein kinase C, or if the stimulation in N-glycosylation activity is a secondary effect of the primary activation process.
Research Organization:
Univ. of Kentucky College of Medicine, Lexington
OSTI ID:
6156634
Report Number(s):
CONF-870644-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DNA REPLICATION
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ESTERS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MANNOSE
MATERIALS
METABOLISM
MICE
MONOSACCHARIDES
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RNA
RODENTS
SACCHARIDES
SOMATIC CELLS
SYNERGISM
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES