Dose determinations for waterborne 2,5,2',5'-(/sup 14/C)tetrachlorobiphenyl and related pharmacokinetics in two species of trout (Salmo gairdneri and Salvelinus fontinalis): a mass-balance approach

Journal Article · Fri Apr 01 04:00:00 EST 1983 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(83)90002-9· OSTI ID:5095797

McKim, J M; Heath, E M

A mass-balance study was undertaken to evaluate the accuracy of dose determinations of waterborne 2,5,2',5'-(/sup 14/C)tetrachlorobiphenyl (TCB) made on transected brook trout (Salvelinus fontinalis) and rainbow trout (Salmo gairdneri) and to determine any pharmacokinetic differences between the two species. The total calculated (/sup 14/C)TCB absorbed by brook (17.8 micrograms) and rainbow (24.5 micrograms) trout was compared to the actual body burden measurements of (/sup 14/C)TCB for brook (17.4 micrograms) and rainbow (25.6 micrograms) trout; the latter measurements also included excretory losses through the urine, feces, and across the gill surface. Approximately 1% of the total dose was excreted of which 75% was in the urine and 25% in the feces. The agreement between the whole body burden measurements of (/sup 14/C)TCB and the total calculated micrograms of (/sup 14/C)TCB absorbed was within 10% in both species. Mass-balance measurements were converted to dose by dividing by fish weight. Mean calculated and measured doses were 31.4 and 30.4 micrograms/kg/48 hr for brook trout and 32.3 and 33.6 micrograms/kg/48 hr for rainbow trout. No species differences were seen in either calculated or measured doses or in total radioactivity excreted. Respiratory function between the two species was similar except for a significantly higher mean ventilation rate for brook trout (84 +/- 14/min) than for rainbow trout (65 +/- 4/min). There were also no fluctuations noted in either respiratory function of (/sup 14/C)TCB uptake efficiency across the gills over the 48-hr exposure period. These studies demonstrated the ability to accurately calculate a water dose in micrograms per kilogram per hour for individual fish that could be directly compared to other fish species or to mammals.

• Research Organization: US Environmental Protection Agency, Duluth, MN
• OSTI ID: 5095797
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 68:2; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY BURDEN
BODY FLUIDS
CARBON 14 COMPOUNDS
CHLORINATED AROMATIC HYDROCARBONS
CLEARANCE
CONTAMINATION
EXCRETION
FECES
FISHES
HALOGENATED AROMATIC HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MASS BALANCE
MATERIALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
TOXICITY
TRACER TECHNIQUES
TROUT
UPTAKE
URINE
VERTEBRATES
WASTES