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Effects of irradiation on endothelial cell-polymorphonuclear leukocyte interactions

Journal Article · · J. Appl. Physiol.; (United States)
OSTI ID:5091814
; ;
Prominent early effects of irradiation include neutrophilic vasculitis and interstitial inflammation. To examine the role of the endothelium in these events, bovine aortic endothelial cells (EC) were irradiated (5 Gy) under ambient conditions followed by measurements of neutrophil chemotaxis toward conditioned media and adherence to EC. Neutrophil chemotactic activity increased at 4, 24, and 72 h in both the sham-treated (4.2 +/- 2.5, 15.2 +/- 4.8, and 20.0 +/- 2.7 microns, respectively) and irradiated EC-conditioned media (5.0 +/- 2.1, 18.7 +/- 4.5, and 24.1 +/- 3.4 microns, respectively), and the difference between them was significant at 72 h. The chemoattractant was trypsin sensitive, heat resistant, and chemotactic. It was not present in the EC sonicate. Adherence of neutrophils to EC that were irradiated 4 h earlier (19.3 +/- 4.2%) increased compared with controls (11.1 +/- 2.4%) and was similar to EC pretreated with zymosan-activated serum (22.0 +/- 4.0%), which is a potent inducer of adherence. Thus, following irradiation, bovine aortic EC have greater neutrophil chemotactic activity in their media and are more adherent to polymorphonuclear leukocytes.
OSTI ID:
5091814
Journal Information:
J. Appl. Physiol.; (United States), Journal Name: J. Appl. Physiol.; (United States) Vol. 6; ISSN JAPYA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560152 -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADSORPTION
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD VESSELS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CATTLE
DOMESTIC ANIMALS
EARLY RADIATION EFFECTS
ENDOTHELIUM
INFLAMMATION
LEUKOCYTES
MAMMALS
MATERIALS
NEUTROPHILS
ORGANS
PATHOLOGICAL CHANGES
RADIATION EFFECTS
RUMINANTS
SORPTION
SURFACE PROPERTIES
SYMPTOMS
TISSUES
VEINS
VERTEBRATES