Chemotaxis of large granular lymphocytes
Journal Article
·
· J. Immunol.; (United States)
OSTI ID:6801214
The hypothesis that large granular lymphocytes (LGL) are capable of directed locomotion (chemotaxis) was tested. A population of LGL isolated from discontinuous Percoll gradients migrated along concentration gradients of N-formyl-methionyl-leucyl-phenylalanine (f-MLP), casein, and C5a, well known chemoattractants for polymorphonuclear leukocytes and monocytes, as well as interferon-..beta.. and colony-stimulating factor. Interleukin 2, tuftsin, platelet-derived growth factor, and fibronectin were inactive. Migratory responses were greater in Percoll fractions with the highest lytic activity and HNK-1/sup +/ cells. The chemotactic response to f-MLP, casein, and C5a was always greater when the chemoattractant was present in greater concentration in the lower compartment of the Boyden chamber. Optimum chemotaxis was observed after a 1 hr incubation that made use of 12 ..mu..m nitrocellulose filters. LGL exhibited a high degree of nondirected locomotion when allowed to migrate for longer periods (> 2 hr), and when cultured in vitro for 24 to 72 hr in the presence or absence of IL 2 containing phytohemagluttinin-conditioned medium. LGL chemotaxis to f-MLP could be inhibited in a dose-dependent manner by the inactive structural analog CBZ-phe-met, and the RNK tumor line specifically bound f-ML(/sup 3/H)P, suggesting that LGL bear receptors for the chemotactic peptide.
- Research Organization:
- Univ. of Manitoba, Winnipeg
- OSTI ID:
- 6801214
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:1; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL PROPERTIES
HYDROGEN ISOTOPES
INFLAMMATION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
MATERIALS
MEMBRANE PROTEINS
MIGRATION
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SEDIMENTATION
SYMPTOMS
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL PROPERTIES
HYDROGEN ISOTOPES
INFLAMMATION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
MATERIALS
MEMBRANE PROTEINS
MIGRATION
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SEDIMENTATION
SYMPTOMS
TRACER TECHNIQUES
TRITIUM
YEARS LIVING RADIOISOTOPES