Human cord blood suppressor T lymphocytes. II. Characterization of inducer of suppressor cells

Journal Article · Sun Jun 01 04:00:00 EDT 1986 · Am. J. Reprod. Immunol. Microbiol.; (United States)

DOI:https://doi.org/10.1111/j.1600-0897.1986.tb00027.x· OSTI ID:5091793

Cheng, H; Delespesse, G

Previously, we reported an antigen nonspecific inducer of T suppressor cell factor (TisF) produced by cord blood mononuclear cells (MNC) in 48-hr, two-way mixed lymphocyte cultures (MLC). The target of this factor was a radiosensitive, T4+ (T8-) adult suppressor T cell subset. The cellular origin of this TisF was examined in the present study. IgG production by pokeweed mitogen (PWM)-stimulated adult MNC was used as an assay for TisF activity. It was found that TisF-producing cells formed rosettes with sheep erythrocytes (E+) and were independent of adherent cells (AC) in the production of TisF. They were resistant to irradiation (2500 rads) and phenotypic characterization with T cell reactive monoclonal antibodies indicated that they resided in the T8- (T4+) population. Furthermore, both TQ1- and TQ1+ cells were required for the production of TisF activity and such activity could not be reconstituted by supernatants from TQ1- MLC and TQ1+ MLC. These results indicate that the production of TisF is dependent upon interactions between radioresistant E+, T8-, TQ1- and radioresistant E+, T8-, TQ1+ cells.

• Research Organization: Univ. of Manitoba, Winnipeg
• OSTI ID: 5091793
• Journal Information: Am. J. Reprod. Immunol. Microbiol.; (United States), Journal Name: Am. J. Reprod. Immunol. Microbiol.; (United States) Vol. 2; ISSN AJRME
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
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CONNECTIVE TISSUE CELLS
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IMMUNOSUPPRESSION
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LEUKOCYTES
LYMPHOCYTES
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RADIOSENSITIVITY
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