Distribution of sensitivity to 4-nitroquinoline 1-oxide among Japanese lymphoblastoid cell lines
Journal Article
·
· Bulletin of Environmental Contamination and Toxicology; (United States)
- Kyushu Univ., Fukuoka (Japan)
- Nakamura Gakuen Coll., Fukuoka (Japan) Nakamura Junior Coll., Fukuoka (Japan)
The processes through which the UV-mimic chemical carcinogen, 4-nitroquinoline 1-oxide (4NQO), leads to the DNA lesions are well characterized in E. coli, where the formation of stable 4NQO-purine adducts is critical. The DNA excision-repair mechanisms similar to those for E. coli occur in normal human cells. Xeroderma pigmentosum (XP) is an example of a rare recessive autosomal skin disorder which is characterized biochemically as a DNA repair-deficient disease. The fluorescein diacetate (FDA) method was recently used to determine the sensitivity of lymphoblastoid cell lines 4NQO. Viable cells take up, non-fluorescent chemical, FDA and convert it to, a fluorescent molecule, fluorescein by intracellular esterases. DNA damage produced by 4NQO could be evaluated on the basis of the cell lethality by this FDA method. In the present study the authors describe the distribution of sensitivity to 4NQO among lymphoblastoid cell lines established from Japanese.
- OSTI ID:
- 5085842
- Journal Information:
- Bulletin of Environmental Contamination and Toxicology; (United States), Journal Name: Bulletin of Environmental Contamination and Toxicology; (United States) Vol. 46:1; ISSN 0007-4861; ISSN BECTA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ANIMAL CELLS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
DNA REPAIR
DYES
FLUORESCEIN
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
MOLECULAR BIOLOGY
NITRO COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
POLYCYCLIC NITRO COMPOUNDS
POLYPHENOLS
PYRIDINES
QUINOLINES
REPAIR
SENSITIVITY
TUMOR CELLS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ANIMAL CELLS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
DNA REPAIR
DYES
FLUORESCEIN
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
MOLECULAR BIOLOGY
NITRO COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
POLYCYCLIC NITRO COMPOUNDS
POLYPHENOLS
PYRIDINES
QUINOLINES
REPAIR
SENSITIVITY
TUMOR CELLS