DNA repair with purines and pyrimidines in radiation- and carcinogen- damaged normal and xeroderma pigmentosum human cells

Journal Article · Thu Feb 01 04:00:00 EST 1973 · Cancer Res., v. 33, pp. 362-369

OSTI ID:4422854

Cleaver, J E

Repair replication of DNA in cells exposed to uv, x rays, or chemical carcinogens involves incorporation of both purine and pyrimidine precursors. Previous reports stating that purines are not involved in repair must be due to insufficient resolution in the reported experiments. Hypoxanthine was more efficiently incorporated by repair replication than other precursors such as adenine, deoxyadenosine, and guanine. Hydroxyurea did not inhibit hypoxanthine incorporation by repair replication. Cells from patients with the hereditary high-cancer disease xeroderma pigmentosum show reduced repair after exposure to uv, 4-nitroquinoline 1-oxide, and 1,3-bis(2-chloroethyl)-1-nitrosourea; they show normal repair after exposure to agents such as N-methyl-N'-nitroN- nitrosoguanidine. These results allow agents to be classified according to whether or not xeroderma pigmentosum cells respond normally to them and illustrate the values and limitations of the use of xeroderma pigmentosum cells or measurements of DNA repair as test systems for carcinogens. (auth)

• Research Organization: Univ. of California, San Francisco
• NSA Number: NSA-29-000668
• OSTI ID: 4422854
• Report Number(s): UCSF--10-P-2-169
• Journal Information: Cancer Res., v. 33, pp. 362-369, Journal Name: Cancer Res., v. 33, pp. 362-369; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

*DNA-- STRAND BREAKS
*STRAND BREAKS-- BIOLOGICAL REPAIR
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARCINOGENS
IRRADIATION
MAN
N48510* --Life Sciences--Radiation Effects on Animals--Man
PHOTON BEAMS
PURINES
PYRIMIDINES
SKIN
SKIN DISEASES
SOMATIC CELLS
ULTRAVIOLET RADIATION
X RADIATION