Repair replication and sister chromatid exchanges as indicators of excisable and nonexcisable damage in human (xeroderma pigmentosum) cells

Journal Article · Fri Jul 01 04:00:00 EDT 1977 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287397709529538· OSTI ID:5088703

Cleaver, J E

Alkylating agents cause damage to DNA which can be repaired by excision repair (repair replication) and which induces sister chromatid exchanges (SCEs). During early times after exposure (within 5 hr) normal and xeroderma pigmentosum (XP) cells perform similar amounts of repair replication. At later times (after 20 hr) normal cells have completed repair replication but XP cells continue at a lower rate. This slowly repaired component of alkylation damage appears to be related to the higher frequencies of SCEs induced by alkylating agents in XP cells compared to normal cells. Therefore, whereas repair replication is an indicator of excised damage, SCEs may be indicators of unexcised damage and may show better correlations with the potentially mutagenic and carcinogenic effects of exposure to environmental agents.

• Research Organization: Univ. of California, San Francisco
• OSTI ID: 5088703
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 2:6; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
560306 -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANIMAL CELLS
ANTIMITOTIC DRUGS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARCINOGENESIS
CHROMATIDS
DNA
DRUGS
MUTAGENESIS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
RECOVERY
REPAIR