Capillary zone electrophoresis: instrumentation and use with nonaqueous solvents

Technical Report · Sun Apr 01 04:00:00 EST 1984

OSTI ID:5076962

David, P A; Pellechia, P J; Manning, D L; Maskarinec, M P

Capillary zone electrophoresis was performed in open tubular fused silica capillaries as small as 60 ..mu..m inside diameter at applied voltages up to 30 kV. On-column detectors based on ultraviolet absorbance and molecular fluorescence were designed and constructed. Migration of polynuclear aromatic hydrocarbons (PNA) was observed in aqueous as well as nonaqueous media. Electroosmotic flow allowed separation of compounds by differential migration. Retention times decreased as medium polarity increased. Retention times ranged from 2 to 15 minutes for PNAs. Efficiency was considerably greater in aqueous media although resolution was greater in nonaqueous media.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5076962
• Report Number(s): ORNL/TM-9141; ON: DE84010617
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
AQUEOUS SOLUTIONS
AROMATICS
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
DISPERSIONS
ELECTROPHORESIS
HYDROCARBONS
MIXTURES
NONAQUEOUS SOLVENTS
ORGANIC COMPOUNDS
ORGANS
POLYCYCLIC AROMATIC HYDROCARBONS
SOLUTIONS
SOLVENTS